Antibody Neutralization of CXCL10 in Vivo Is Dependent on Binding to Free and Not Endothelial-bound Chemokine: IMPLICATIONS FOR THE DESIGN OF A NEW GENERATION OF ANTI-CHEMOKINE THERAPEUTIC ANTIBODIES.
To enhance our understanding of properties that confer profitable inhibition of chemokines in vivo, we analyzed anti-murine CXCL10 monoclonal antibodies (mAb) having totally different traits. 1B6 displayed potent inhibition of cell recruitment in vitro with an IC50 of 0.5 nm however demonstrated little efficacy in varied animal fashions of human illness. Quite the opposite, 1F11 confirmed efficacy in a number of fashions of irritation but was...