Use: sufficient for 2500 colourimetric tests
Detection method: colorimetric
Storage temperature: 2-8 ° C
Phosphate is one of the most abundant minerals in the human body and contains approximately 1% of total body weight. Phosphate homeostasis is maintained by various regulators such as parathyroid hormone (PTH), calcitriol, and phosphating. Maintaining phosphate homeostasis is essential for several biological processes, such as energy metabolism, cell signalling, regulation of protein synthesis, skeletal development, and bone integrity. † An adequate level of phosphate is required for the apoptosis process of mature chondrocytes in the growth plate. . Phosphate deficiency is associated with bone pathology and clinical disease. †
The Malachite Green Phosphate Assay Kit has been used for phosphate quantification and steady-state kinetics. The Malachite Green Phosphate Assay Kit has been used to measure the release of free orthophosphate in the following applications:
Phosphatase assays: release of phosphate from peptides, proteins, or small molecule substrates.
Lipase Assays: Phospholipid Phosphate Release
Nucleoside triphosphatase assays: release of phosphate from a nucleoside triphosphate (ATP, GTP, TTP, CTP, etc.).
Quantification of phosphate in phospholipids, proteins and DNA, etc.
Drug Discovery: High Throughput Screening for Phosphatase Inhibitors.
Features and Benefits
Compatible with high-performance handling systems.
Suitable for the determination of phosphate in a variety of biological and environmental samples.
Malachite green is a basic dye that belongs to the triphenylmethane group. It is used in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate. †† The Malachite Green Phosphate Assay Kit is based on the quantification of the green complex formed between malachite green, molybdate, and free orthophosphate. The rapid colour formation of the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader.
Non-radioactive colourimetric test kits have been optimized for superior sensitivity and long shelf life. The assay is simple and fast, with a single additional step for the determination of phosphate. Assays can be performed in tubes, cuvettes, or multi-well plates. Assays can be conveniently performed in 96- and 384-well plates for screening high-throughput enzyme inhibitors.