Skip to content
International Academic Journals

International Academic Journals

Plant species genetics, cell culture, Elisa kits, antibodies

  • Home
  • anti-Human
  • Biotin
  • beads
  • gel
  • Contact Us
  • Contact us
  • Terms and Conditions
Close Button

Evaluation of the INDICAID COVID-19 Rapid Antigen Test in Symptomatic Populations and Asymptomatic Community Testing

| GregoryGregory | 0 Comment
As the COVID-19 pandemic progresses, there is an increasing need for rapid, accessible assays for SARS-CoV-2 detection. We present a clinical evaluation and real-world implementation of the INDICAID COVID-19 rapid antigen test (INDICAID rapid test).
A multisite clinical evaluation of the INDICAID rapid test using prospectively collected nasal (bilateral anterior) swab samples from symptomatic subjects was performed. The INDICAID rapid test demonstrated a positive percent agreement (PPA) and negative percent agreement (NPA) of 85.3% (95% confidence interval [95% CI], 75.6% to 91.6%) and 94.9% (95% CI, 91.6% to 96.9%), respectively, compared to laboratory-based reverse transcriptase PCR (RT-PCR) using nasal specimens. The INDICAID rapid test was then implemented at COVID-19 outbreak screening centers in Hong Kong as part of a testing algorithm (termed “dual-track”) to screen asymptomatic individuals for prioritization for confirmatory RT-PCR testing.
In one approach, preliminary positive INDICAID rapid test results triggered expedited processing for laboratory-based RT-PCR, reducing the average time to confirmatory result from 10.85 h to 7.0 h. In a second approach, preliminary positive results triggered subsequent testing with an onsite rapid RT-PCR, reducing the average time to confirmatory result to 0.84 h. In 22,994 asymptomatic patients, the INDICAID rapid test demonstrated a PPA of 84.2% (95% CI, 69.6% to 92.6%) and an NPA of 99.9% (95% CI, 99.9% to 100%) compared to laboratory-based RT-PCR using combined nasal/oropharyngeal specimens. The INDICAID rapid test Indicaid has excellent performance compared to laboratory-based RT-PCR testing and, when used in tandem with RT-PCR, reduces the time to confirmatory positive result.

IMPORTANCE

  • Laboratory-based RT-PCR, the current gold standard for COVID-19 testing, can require a turnaround time of 24 to 48 h from sample collection to result. The delayed time to result limits the effectiveness of centralized RT-PCR testing to reduce transmission and stem potential outbreaks.
  • To address this, we conducted a thorough evaluation of the INDICAID COVID-19 rapid antigen test, a 20-minute rapid antigen test, in both symptomatic and asymptomatic populations. The INDICAID rapid test demonstrated high sensitivity and specificity with RT-PCR as the comparator method.
  • A dual-track testing algorithm was also evaluated utilizing the INDICAID rapid test to screen for preliminary positive patients, whose samples were then prioritized for RT-PCR testing. The dual-track method demonstrated significant improvements in expediting the reporting of positive RT-PCR test results compared to standard RT-PCR testing without prioritization, offering an improved strategy for community testing and controlling SARS-CoV-2 outbreaks.
  • The global SARS-CoV-2 pandemic has continued despite implementation of significant public health measures (1). Over 155 million worldwide cases of COVID-19 and over 3 million COVID-19 deaths have been reported as of May 2021. Rapid identification of SARS-CoV-2 infection, patient isolation, and contact tracing are essential for disease containment (2).

The current gold standard for detecting SARS-CoV-2 is reverse transcriptase PCR (RT-PCR) (3).

While RT-PCR can detect nucleic acids from SARS-CoV-2 with high sensitivity, RT-PCR requires equipment and special training and can take days for results to be available following sample collection (4). Due to the transmissibility of SARS-CoV-2 (basic reproductive number R0 of 2.87), long turnaround times for results may lead to a high number of avoidable transmissions .
In contrast, lateral flow immunoassays (LFAs) are an inexpensive testing solution that can be used at point-of-care settings, do not require laboratory equipment, and can generate results quickly. However, the performance of LFA-based SARS-CoV-2 rapid antigen tests in community testing settings can vary significantly.
In this study, we evaluated the clinical performance of the LFA-based INDICAID COVID-19 rapid antigen test (INDICAID rapid test) by PHASE Scientific International Ltd.
A prospective multisite clinical study was performed in symptomatic patient populations in point-of-care (POC) community testing sites in the United States. The performance of the INDICAID rapid test was also evaluated in COVID-19 outbreak screening centers in Hong Kong as a part of an algorithm testing approach (termed “dual-track”) to screen for COVID-19-positive patients prior to RT-PCR testing in asymptomatic patient populations.

Prospective multisite clinical evaluation of the INDICAID COVID-19 rapid antigen test.

San Francisco and Oakland population characteristics.
In total, 83 participants with at least two COVID-19 symptoms were enrolled at the San Francisco, CA and Oakland, CA sites. Two participants were excluded from the analysis due to lost samples during transport for RT-PCR. Of the 81 participant specimens analyzed, 44.4% were from female participants (Table S1), with a median age of 32 years (interquartile range [IQR], 25, 44). The most frequently reported symptoms were muscle/body ache (61.7%), congestion/runny nose (60.5%), fatigue (56.8%), and headache (53.1%). The breakdown of duration of symptoms was 1 to 2 days in 37 participants (45.7%), 3 to 4 days in 38 participants (46.9%), and 5 days in 6 participants (7.4%).
San Fernando population characteristics.
In total, 270 participants with at least one COVID-19 symptom were enrolled at the San Fernando, CA site. Two participants were excluded from the analysis due to lost or spilled samples during transport for RT-PCR. Of the 268 participant specimens analyzed, 52.6% were from female participants (Table S2), and the median age was 35 years (IQR, 24, 50). The most frequently reported symptoms were sore throat (60.8%), headache (60.1%), congestion/runny nose (59.0%), and cough (54.9%). The distribution of duration of symptoms was 1 to 2 days in 109 participants (40.7%), 3 to 4 days in 127 participants (47.4%), and 5 days in 32 participants (11.9%).
Performance of the INDICAID COVID-19 rapid antigen test in symptomatic patients.
Of the total 329 participant specimens included in the analyses, 75 tested positive with the comparator laboratory-based RT-PCR test. The mean cycle threshold value was 20.79 ± 6.39.
The INDICAID rapid test demonstrated a PPA of 85.3% (95% confidence interval [95% CI], 75.6% to 91.6%) and an NPA of 94.9% (95% CI, 91.6% to 96.9%) when sample collection was conducted by a health care professional. There was a total of 11 false-negative INDICAID results that were not concordant with RT-PCR results; the mean threshold cycle (CT) value of the false-negative INDICAID specimens was 32.56 ± 4.59.
Tags: rapid, rapid antigen test, rapid card login, rapid city journal, rapid city sd, rapid covid test near me, rapid covid testing, rapid covid testing near me, rapid identity ncedcloud, rapid paycard, rapid pcr test near me, rapid recon, rapid response, rapid sos login, rapid testing near me, rapid typing, rapidentity, rapidgator, rapididentity login, rapido, rapids appointment scheduler, rapidsos, rapidsos portal/login, rapidvisa

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Post navigation

PREVIOUS Previous post: Tumor cell-derived SPON2 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by activating PYK2 in CRC
NEXT Next post: Clinical assessment of the Roche SARS-CoV-2 rapid antigen test

Related Post

Pan-methylarginine antibody generation using PEG linked GAR motifs as antigensPan-methylarginine antibody generation using PEG linked GAR motifs as antigens

Arginine methylation is a prevalent posttranslational modification which is deposited by a household of protein arginine methyltransferases (PRMTs), and is present in three totally different types in mammalian cells: monomethylarginine

READ MOREREAD MORE
Development of a nanobody-based competitive ELISA for efficiently and specifically detecting antibodies against genotype 2 porcine reproductive and respiratory syndrome viruses

Development of a nanobody-based competitive ELISA for efficiently and specifically detecting antibodies against genotype 2 porcine reproductive and respiratory syndrome virusesDevelopment of a nanobody-based competitive ELISA for efficiently and specifically detecting antibodies against genotype 2 porcine reproductive and respiratory syndrome viruses

Porcine reproductive and respiratory syndrome virus (PRRSV) an infection causes appreciable financial loss to the worldwide pig business. Environment friendly detection assay is essential for the prevention of the virus

READ MOREREAD MORE
A generic sample preparation method for the multiplex analysis of seven therapeutic monoclonal antibodies in human plasma or serum with liquid chromatography-tandem mass spectrometry

A generic sample preparation method for the multiplex analysis of seven therapeutic monoclonal antibodies in human plasma or serum with liquid chromatography-tandem mass spectrometryA generic sample preparation method for the multiplex analysis of seven therapeutic monoclonal antibodies in human plasma or serum with liquid chromatography-tandem mass spectrometry

Because of the rising variety of therapeutic monoclonal antibodies (mAbs) used within the clinic, there’s an rising want for sturdy analytical strategies to quantify complete mAb concentrations in human plasma

READ MOREREAD MORE
International Academic Journals
International Academic Journals

Categories

  • Abt 199
  • anti cd68
  • anti-Human
  • Azd 0530
  • beads
  • Biotin
  • Bird
  • Blog
  • Bq 788
  • Caspase 1 Assay
  • Cck 8 Kit
  • Dylight 549
  • e. coli
  • Fgf 21 Elisa
  • fusion
  • gel
  • Glp 1 Elisa
  • Goat
  • Gsk 872
  • Gw 0742
  • Hamster
  • Igf 1 Elisa
  • Il 10 Elisa
  • Keratin 14 Antibody
  • Keratin 18
  • List Of Journal
  • Mac 2 Antibody
  • Mek 162
  • Mk 4827
  • Mouse
  • Mucin 2 Antibody
  • Nkx2 1 Antibody
  • Nkx2 2 Antibody
  • nod2 antibody
  • PCR
  • pecy5
  • peptide
  • Pgc 1 Alpha Antibody
  • Post
  • Rat
  • Ro 48 8071
  • rps20
  • Sheep
  • Spoligotyping
  • Standard
  • Stemregenin 1
  • Transglutaminase 3
  • urea assay kit
  • Xav 939
  • Yeast

Recent Posts

  • Mouse Translocator Protein (TSPO) ELISA: Comprehensive Insights into Mitochondrial Function and Inflammation
  • Glial Cell Line-Derived Neurotrophic Factor (GDNF): A Comprehensive Scientific Overview
  • Goat Anti-tdTomato DyLight Antibody: A Comprehensive Tool for Fluorescent Protein Detection in Imaging and Cell Sorting
  • Streptavidin Magnetic Beads: Applications, Mechanisms, and Advantages in Molecular Biology
  • The Technical Principles Behind EVO HS Super-Fidelity DNA Polymerase

Tags

acetyl lysine antibody ae1 ae3 antibody akt antibody alexa fluor 488 goat anti rabbit annexin v-fitc apoptosis detection kit anti flag magnetic beads anti igg antibody assay kit definition biotin hrp bl21 de3 cells bmal1 antibody cas9 protein molecular weight cdna synthesis dapi blue d dimer elisa dsred antibody fetal bovine serum cell culture gelatin coated slides histamine elisa mac-2 antibody maxi prep protocol muc5ac antibody orbital shakers pan cadherin antibody pappen pcr cleanup peptide bond peptide bond definition peptides definition peptides for sale peptide volume essence la gi peptide volume essence webtretho plasmid purification kit plate shaker protein molecular weight marker quikchange ii site directed mutagenesis kit raybio cytokine array sds running buffer recipe sure competent cells sybr green i talon beads transfer membranes trap antibody vegfr1 antibody western blot kit

Contact Us

[contact-form-7 id=”13″ title=”Contact form 1″]

Categories

  • Abt 199
  • anti cd68
  • anti-Human
  • Azd 0530
  • beads
  • Biotin
  • Bird
  • Blog
  • Bq 788
  • Caspase 1 Assay
  • Cck 8 Kit
  • Dylight 549
  • e. coli
  • Fgf 21 Elisa
  • fusion
  • gel
  • Glp 1 Elisa
  • Goat
  • Gsk 872
  • Gw 0742
  • Hamster
  • Igf 1 Elisa
  • Il 10 Elisa
  • Keratin 14 Antibody
  • Keratin 18
  • List Of Journal
  • Mac 2 Antibody
  • Mek 162
  • Mk 4827
  • Mouse
  • Mucin 2 Antibody
  • Nkx2 1 Antibody
  • Nkx2 2 Antibody
  • nod2 antibody
  • PCR
  • pecy5
  • peptide
  • Pgc 1 Alpha Antibody
  • Post
  • Rat
  • Ro 48 8071
  • rps20
  • Sheep
  • Spoligotyping
  • Standard
  • Stemregenin 1
  • Transglutaminase 3
  • urea assay kit
  • Xav 939
  • Yeast

Recent Posts

  • Mouse Translocator Protein (TSPO) ELISA: Comprehensive Insights into Mitochondrial Function and Inflammation
  • Glial Cell Line-Derived Neurotrophic Factor (GDNF): A Comprehensive Scientific Overview
  • Goat Anti-tdTomato DyLight Antibody: A Comprehensive Tool for Fluorescent Protein Detection in Imaging and Cell Sorting
  • Streptavidin Magnetic Beads: Applications, Mechanisms, and Advantages in Molecular Biology
  • The Technical Principles Behind EVO HS Super-Fidelity DNA Polymerase

Quick Links

  • Contact Us
  • Contact us
  • International Academic Journals
  • Join Us
  • Terms and Conditions

VW Wellness Coach WordPress Theme By VWThemes

Scroll Up