Dynamic integration of enteric neural stem cells in ex vivo organotypic colon cultures

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Dynamic integration of enteric neural stem cells in ex vivo organotypic colon cultures

Enteric neural stem cells (ENSC) have been recognized as a attainable therapy for enteric neuropathies. After in vivo transplantation, ENSC and their derivatives have been proven to engraft inside colonic tissue, migrate and populate endogenous ganglia, and functionally combine with the enteric nervous system. Nonetheless, the mechanisms underlying the combination of donor ENSC, in recipient tissues, stay unclear. Subsequently, we aimed to look at ENSC integration utilizing an tailored ex vivo organotypic tradition system. Donor ENSC have been obtained from Wnt1cre/+;R26RYFP/YFP mice permitting particular labelling, choice and fate-mapping of cells.

YFP+ neurospheres have been transplanted to C57BL6/J (6-8-week-old) colonic tissue and maintained in organotypic tradition for as much as 21 days. We analysed and quantified donor cell integration inside recipient tissues at 7, 14 and 21 days, together with assessing the structural and molecular penalties of ENSC integration. We discovered that organotypically cultured tissues have been properly preserved as much as 21-days in ex vivo tradition, which allowed for evaluation of donor cell integration after transplantation. Donor ENSC-derived cells built-in throughout the colonic wall in a dynamic style, throughout a three-week interval.

Following transplantation, donor cells displayed two integrative patterns; longitudinal migration and medial invasion which allowed donor cells to populate colonic tissue. Furthermore, important remodelling of the intestinal ECM and musculature occurred upon transplantation, to facilitate donor cell integration inside endogenous enteric ganglia. These outcomes present crucial proof on the timescale and mechanisms, which regulate donor ENSC integration, inside recipient intestine tissue, that are vital concerns sooner or later medical translation of stem cell therapies for enteric illness.

Learning human ageing is of accelerating significance because of the worldwide ageing inhabitants. Nonetheless, it faces the problem of prolonged experiments to supply an ageing phenotype. Usually, to recreate the hallmarks of ageing requires complicated empirical situations that may confound knowledge interpretation. Certainly, many research use complete organisms with comparatively quick life spans, which can have little, or restricted, relevance to human ageing. There was intensive use of cell traces to check ageing in human somatic cells, however the modelling of human neuronal ageing is considerably extra complicated in vitro.

Cladosporols A and B, two pure peroxisome proliferator-activated receptor gamma (PPARγ) agonists, inhibit adipogenesis in Three T3-L1 preadipocytes and trigger a conditioned-tradition-medium-dependent arrest of HT-29 cell proliferation

Weight problems and kind 2 diabetes mellitus, that are widespread all through the world, require therapeutic interventions focused to unravel medical issues (insulin resistance, hyperglycaemia, dyslipidaemia and steatosis). A number of pure compounds at the moment are a part of the therapeutic repertoire developed to higher handle these pathological situations. Cladosporols, secondary metabolites from the fungus Cladosporium tenuissimum, have been characterised for his or her capacity to regulate cell proliferation in human colon most cancers cell traces by means of peroxisome proliferator-activated receptor gamma (PPARγ)-mediated modulation of gene expression. Right here, we report knowledge regarding the capacity of cladosporols to manage the differentiation of murine Three T3-L1 preadipocytes.

We confirmed that cladosporol A and B inhibited the storage of lipids in Three T3-L1 mature adipocytes, whereas their administration didn’t have an effect on the proliferative capacity of preadipocytes. Furthermore, each cladosporols downregulated mRNA and protein ranges of early (C/EBPα and PPARγ) and late (aP2, LPL, FASN, GLUT-4, adiponectin and leptin) differentiation markers of adipogenesis. Lastly, we discovered that proliferation and migration of HT-29 colorectal most cancers cells have been inhibited by conditioned medium from cladosporol-treated Three T3-L1 cells in contrast with the preadipocyte conditioned medium.

 

Co-tradition with mouse embryonic fibroblasts improves upkeep of metabolic operate of human small hepatocyte progenitor cells

Derivation and tradition of small hepatocyte progenitor cells (SHPCs) able to proliferating in vitro has been described in rodents and not too long ago in people. These cells are able to engrafting in injured livers, nonetheless, they show de-differentiated morphology and decreased xenobiotic metabolism exercise in tradition over passages. Right here we report that SHPCs derived from grownup main human hepatocytes (PHHs) and cultured on mouse embryonic fibroblasts (MEFs) not solely show differentiated morphology and exhibit gene expression profiles much like grownup PHHs, however importantly, they keep their phenotype over a number of passages.

Additional, in contrast to earlier stories, the place intensive manipulations of tradition situations are required to transform SHPCs to metabolically purposeful hepatocytes, SHPCs in our co-culture system preserve expression of xenobiotic metabolism-associated genes. We present that SHPCs in co-culture are in a position to carry out xenobiotic metabolism at charges equal to their father or mother PHHs as evidenced by the metabolism of acetaminophen to all of its main metabolites. In abstract, we current an improved co-culture system that permits era of SHPCs from grownup PHHs that preserve their differentiated phenotype over a number of passages. Our findings can be helpful for growth of restricted PHHs to be used in research of drug metabolism and toxicity testing.

Sphingomonas sp. Shah is a bacterium that was first remoted from mammalian cell cultures. In keeping with ribotyping knowledge it is vitally a lot homologous to the clinically vital pathogen Sphingomonas paucimobilis, which has generated pseudo-outbreaks. Utilizing a tissue tradition system, Sphingomonas sp. Shah was found to induce apoptosis in human lung epithelial carcinoma. Apoptosis of contaminated cells was decided by quite a few standards together with (1) visible alterations in mobile morphology

Chicken Kit ligand (KITLG) ELISA Kit
abx511857-96tests 96 tests
EUR 1093.2

Dog Kit ligand (KITLG) ELISA Kit
abx511858-96tests 96 tests
EUR 1093.2

Mouse Kit ligand (KITLG) ELISA Kit
abx511860-96tests 96 tests
EUR 801.6

Pig Kit ligand (KITLG) ELISA Kit
abx511861-96tests 96 tests
EUR 1093.2

Rat Kit ligand (KITLG) ELISA Kit
abx511862-96tests 96 tests
EUR 801.6

Human Kit Ligand, KitLG ELISA Kit
DEIA141 10 plates
EUR 2994
Description: Human KITLG ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant KITLG in a sandwich ELISA format within the range of 16-2,000 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay KITLG in approximately 1,000 ELISA plate wells

Rat Kit Ligand, Kitlg ELISA Kit
DEIA142 10 plates
EUR 2994
Description: The Human DKK1 ELISA kit is for the quantitative determination of Human DKK1.;This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Rat Kitlg/ Kit ligand ELISA Kit
E0545Ra 1 Kit
EUR 655.2

Rabbit Kit ligand(KITLG) ELISA kit
E04K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kit ligand(KITLG) ELISA kit
E04K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kit ligand(KITLG) ELISA kit
E04K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kit ligand(KITLG) ELISA kit
E01K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kit ligand(KITLG) ELISA kit
E01K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kit ligand(KITLG) ELISA kit
E01K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bovine KITLG/ Kit ligand ELISA Kit
E0164Bo 1 Kit
EUR 860.4

Mouse Kit ligand(KITLG) ELISA kit
E03K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kit ligand(KITLG) ELISA kit
E03K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kit ligand(KITLG) ELISA kit
E03K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human KITLG(Kit ligand ) ELISA Kit
EH0514 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Monkey Kit ligand(KITLG) ELISA kit
E09K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kit ligand(KITLG) ELISA kit
E09K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kit ligand(KITLG) ELISA kit
E09K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human KITLG/ Kit ligand ELISA Kit
E1378Hu 1 Kit
EUR 644.4

Mouse Kitlg/ Kit ligand ELISA Kit
E1654Mo 1 Kit
EUR 655.2

Rat Kit ligand(KITLG) ELISA kit
E02K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kit ligand(KITLG) ELISA kit
E02K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kit ligand(KITLG) ELISA kit
E02K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kit ligand(KITLG) ELISA kit
E08K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kit ligand(KITLG) ELISA kit
E08K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kit ligand(KITLG) ELISA kit
E08K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kit ligand(KITLG) ELISA kit
E07K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kit ligand(KITLG) ELISA kit
E07K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kit ligand(KITLG) ELISA kit
E07K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Kit Ligand (KITLG) Antibody
20-abx006306
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Kit Ligand (KITLG) Antibody
abx122984-100ug 100 ug
EUR 469.2

Kit Ligand (KITLG) Antibody
20-abx125169
  • EUR 594.00
  • EUR 844.80
  • 100 ul
  • 200 ul

Kit Ligand (KITLG) Antibody
20-abx321525
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Kit ligand (KITLG) Antibody
abx330456-100ul 100 ul
EUR 510

Human Kit ligand (KITLG)
1-CSB-EP012376HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Kit ligand(KITLG),partial expressed in E.coli

Kit ligand/KITLG/SCF
E21-D53 10ug
EUR 411.6

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes
SPXPR-KIT 1ML
EUR 1923.18

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS410A-KIT 1 kit (10 rxn)
EUR 798

Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS420A-KIT 1 kit (10 rxn)
EUR 828

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT 10 rxn
EUR 1358.4

Guinea pig Kit ligand(KITLG) ELISA kit
E05K0089-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Kit ligand(KITLG) ELISA kit
E05K0089-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Kit ligand(KITLG) ELISA kit
E05K0089-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kit ligand(KITLG) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Pig Kit ligand (KITLG)
KTE80143-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Pig Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Pig Kit ligand (KITLG)
KTE80143-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Pig Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Pig Kit ligand (KITLG)
KTE80143-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Pig Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Kit ligand (KITLG)
KTE10341-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Bovine Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Kit ligand (KITLG)
KTE20076-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Canine Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Kit ligand (KITLG)
KTE20076-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Canine Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Kit ligand (KITLG)
KTE20076-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Canine Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Kit ligand (KITLG)
KTE10341-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Bovine Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Kit ligand (KITLG)
KTE10341-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Bovine Kit ligand (KITLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT 1 Kit
EUR 966

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT 10 rxn
EUR 1358.4

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT 1 Kit
EUR 183.6

c-kit Ligand/SCF/KITLG
E21-034 10ug
EUR 411.6

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL
SPXMT-KIT 30ML
EUR 237.12

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL
SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT 1 Kit
EUR 5929.2

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE624A-KIT 1 kit
EUR 2558.4

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody
EXOAB-KIT-1 25 ul each
EUR 752.4

vWF Acty. Kit
ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit
ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

PrecisionX Multiplex gRNA Cloning Kit
CAS9-GRNA-KIT 10 rxn
EUR 534

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)
CAS620A-KIT 1 kit
EUR 2582.4
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN300A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT 1 Kit
EUR 5202

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT 1 Kit
EUR 5202

Filtration System 47mm Complete Assembly
5809/KIT EACH
EUR 427.69

mRNAExpress mRNA Synthesis kit (5 reactions)
MR-KIT-1 5 reactions
EUR 1382.4

42 Component Terpene Kit in Methanol
CAN-TERP-KIT 1ML
EUR 287.28

Canadian Pesticide Kit Containing All 6 Mixes
CAN-CAN-KIT 1ML
EUR 758.1

AxioCam Erc5s rev 2, w/Accessory Kit
ZV-Erc5sAC-Kit 1 KIT
EUR 1611
Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control

KITLG ELISA KIT|Human
EF000332 96 Tests
EUR 826.8

Human KitLG ELISA Kit
ELA-E0120h 96 Tests
EUR 988.8

Goat FAS Ligand ELISA kit
E06F0051-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat FAS Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat FAS Ligand ELISA kit
E06F0051-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat FAS Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat FAS Ligand ELISA kit
E06F0051-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat FAS Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CD30 Ligand ELISA kit
E06C0335-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat CD30 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CD30 Ligand ELISA kit
E06C0335-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat CD30 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CD30 Ligand ELISA kit
E06C0335-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat CD30 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)
CASCL9-100A-KIT 1 Kit
EUR 1358.4

pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-520A-KIT 1 kit
EUR 1141

pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-550A-KIT 1 kit
EUR 1141

pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-575A-KIT 1 kit
EUR 1141

pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-599A-KIT 1 kit
EUR 1141

pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-639A-KIT 1 kit
EUR 1141

pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-649A-KIT 1 kit
EUR 1141

pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-659A-KIT 1 kit
EUR 1141

pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-669A-KIT 1 kit
EUR 1162

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit
DLR-GOAT-Hu-48T 48T
EUR 664.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Ghrelin-O-Acyltransferase (GOAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit
DLR-GOAT-Hu-96T 96T
EUR 870
Description: A sandwich quantitative ELISA assay kit for detection of Human Ghrelin-O-Acyltransferase (GOAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit
RDR-GOAT-Hu-48Tests 48 Tests
EUR 706.8

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit
RDR-GOAT-Hu-96Tests 96 Tests
EUR 984

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit
RD-GOAT-Hu-48Tests 48 Tests
EUR 675.6

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit
RD-GOAT-Hu-96Tests 96 Tests
EUR 939.6

pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-619A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-629A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-589A-KIT 1 kit
EUR 1141

pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-609A-KIT 1 kit
EUR 1141

Goat soluble OX40 Ligand ELISA kit
E06S0320-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat soluble OX40 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat soluble OX40 Ligand ELISA kit
E06S0320-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat soluble OX40 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat soluble OX40 Ligand ELISA kit
E06S0320-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat soluble OX40 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CD 40 Ligand ELISA kit
E06C0018-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat CD 40 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CD 40 Ligand ELISA kit
E06C0018-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat CD 40 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CD 40 Ligand ELISA kit
E06C0018-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat CD 40 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

KITLG ELISA Kit (Human) (OKBB00735)
OKBB00735 96 Wells
EUR 606
Description: Description of target: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

KITLG ELISA Kit (Rat) (OKBB00736)
OKBB00736 96 Wells
EUR 606
Description: Description of target: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

KITLG ELISA Kit (Mouse) (OKBB00737)
OKBB00737 96 Wells
EUR 606
Description: Description of target: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

KITLG ELISA Kit (Chicken) (OKEH07311)
OKEH07311 96 Wells
EUR 1420.8
Description: Description of target: ;Species reactivity: Chicken;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 39pg/mL

KITLG ELISA Kit (Dog) (OKEH07312)
OKEH07312 96 Wells
EUR 1420.8
Description: Description of target: ;Species reactivity: Dog;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 39pg/mL

KITLG ELISA Kit (Pig) (OKEH07313)
OKEH07313 96 Wells
EUR 1310.4
Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 39pg/mL

KITLG ELISA Kit (Human) (OKAN04599)
OKAN04599 96 Wells
EUR 950.4
Description: Description of target: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.5 pg/mL

KITLG ELISA Kit (Mouse) (OKEH04054)
OKEH04054 96 Wells
EUR 652.8
Description: Description of target: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

Kitlg ELISA Kit (Rat) (OKEH04516)
OKEH04516 96 Wells
EUR 652.8
Description: Description of target: Soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 15.78 pg/mL

KITLG ELISA Kit (Bovine) (OKEH04606)
OKEH04606 96 Wells
EUR 934.8
Description: Description of target: Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins..;Species reactivity: Bovine;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 30 pg/mL

KITLG ELISA Kit (Bovine) (OKCD05997)
OKCD05997 96 Wells
EUR 1248
Description: Description of target: Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins..;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 5.7pg/mL

KITLG ELISA Kit (Dog) (OKCD05998)
OKCD05998 96 Wells
EUR 1201.2
Description: Description of target: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Dog;Application: ELISA;Assay info: ;Sensitivity: < 6.5pg/mL

KITLG ELISA Kit (Human) (OKCD05999)
OKCD05999 96 Wells
EUR 777.6
Description: Description of target: KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 6.5pg/mL

KITLG ELISA Kit (Pig) (OKCD06001)
OKCD06001 96 Wells
EUR 1248
Description: Description of target: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Pig;Application: ELISA;Assay info: ;Sensitivity: < 6.4pg/mL

KITLG ELISA Kit (Rat) (OKCD06002)
OKCD06002 96 Wells
EUR 981.6
Description: Description of target: soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: < 6.3pg/mL

42 Component Terpene Kit High Level in Methanol
CAN-TERP-KIT-H 1ML
EUR 308.94

Assurance Grade Set of 38 Single-Element Standards 1000 ug/mL (1000 PPM)
ICP-KIT-1 EACH
EUR 1914.06

AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS
AP-STR-KIT-P 1/pk
EUR 865.2
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Goat CXC chemokine ligand 13 ELISA kit
E06C2556-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat CXC chemokine ligand 13 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CXC chemokine ligand 13 ELISA kit
E06C2556-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat CXC chemokine ligand 13 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CXC chemokine ligand 13 ELISA kit
E06C2556-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat CXC chemokine ligand 13 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CXC chemokine ligand 16 ELISA kit
E06C0851-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat CXC chemokine ligand 16 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat CXC chemokine ligand 16 ELISA kit
E06C0851-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat CXC chemokine ligand 16 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

initiation of nuclear marginalization and chromatin compaction condensation, (3) the attendance of a excessive proportion of cells with subG1 DNA content material, and (4) caspase-Three activation. Within the present research we show the induction of apoptosis in mammalian lung epithelial cells upon an infection with Sphingomonas sp. Shah and supply perception into the molecular processes triggering apoptosis.

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